As our understanding of soil biology deepens, there is a growing demand for investigations addressing microbial processes in the earth beneath the topsoil layer, called subsoil. High clay content in subsoils often hinders the recovery of sufficient quantities of DNA as clay particles bind nucleic acids. Here, an efficient and reproducible DNA …

The present study is to explore the optimal extraction parameters, antioxidant activity, and antimicrobial activity of alkaline soluble polysaccharides from rhizome of Polygonatum odoratum.The optimal extraction parameters were determined as the following: NaOH concentration (A) 0.3 M, temperature (B) 80°C, ratio of NaOH to solid …

Root canal stripping is defined as an oblong, vertical perforation that appears especially in the middle section of curved root canals during endodontic treatments with nickel-titanium (Ni-Ti) instruments. Its occurrence may drastically affect the outcome of the treatment, transforming a common otherwise efficient endodontic procedure into a …

Inefficiencies in the extraction kits using large sediment weights could explain this observation with the possibility that humic acids and other contaminants are binding to the silica filters and reducing the concentration of DNA bound to the filters (Lloyd, Macgregor & Teske, 2010). If the latter hypothesis is true, then the obvious ...

Abstract. Eugenol, a phytogenic bioactive component is frequently found in diversified herbal plants possessing well-defined functional attributes. Prominent sources of eugenol are clove, cinnamon, tulsi and pepper. Various extraction methods have been practiced globally for the extraction of eugenol and other nutraceutics from plants.

Extraction Methods: Microwave, Ultrasonic, Pressurized Fluid, Soxhlet Extraction, Etc Komal Patel1, 3Namrata Panchal2, Dr. Pradnya Ingle * ... (A and B are pure and …

Abstract. The aim of this work was to evaluate the technologies effect of cold extraction by centrifugation (CE) and ultrasound-assisted (US-CE) methods without adding water, on the avocado oil yield, nutritional composition, physicochemical characteristics, oxidative stability (oxidation temperature and time, besides activation energy) and ...

Extraction uses the property of solubility to transfer a solute from one phase to another phase. Solid-phase extraction (SPE) is an extractive technique by which compounds that are dissolved or suspended in a liquid mixture are separated from other compounds in the mixture according to their physical and chemical properties.

Extraction #1. Perform a single extraction using approximately 25mL 25 mL of dichloromethane ( CH2Cl2 CH 2 Cl 2, an exact amount is not necessary), as described previously, with the following differences: As …

The aim of the study was to identify the postoperative infection rates after tooth extraction in a university dental clinic and to identify the factors associated with an increased risk for postoperative infection. A retrospective study of case records of patients who underwent tooth extractions at the International Medical University's Oral Health …

Natural orifice specimen extraction surgery has shown advantages in alleviating postoperative pain, improving bowel movements, providing a cosmetic effect, and improving psychological state without adversely affecting cancer outcomes. Different approaches have been classified and summarized for natural orifice specimen …

Extraction of maxillary right first premolar was performed. An open coil spring was placed with respect to the blocked out canine for space creation and midline correction in the maxillary arch [Figure 4]. After sufficient space had been created, the left maxillary canine was bonded and aligned within the arch using active lacebacks. Mandibular ...

OSITEX accompagne plusieurs établissements de Santé dans leurs démarches respectives de dépot de dossier d'investissement DIPISI auprès des instances régionales. Au-delà du dépôt du dossier et du contrôle du respect des pré-requis, notre démarche consiste à assurer l'atteinte des cibles Hopital Numérique conformément aux engagements.

The extraction techniques are the powerful tool to produce extract and purify a wide range of target materials from different samples. They can be either sorbent based or solvent based. Many integrated and sustainable extraction techniques have been developed and employed that make the attention to researchers. Common extraction techniques are ...

ORGANIC EXTRACTION AND SAMPLE PREPARATION 1.0 SCOPE AND APPLICATION 1.1 Method 3500 provides general guidance on the selection of methods used in the ... standard materials or pur chased as certified solutions. The stock solutions used for the calibration standards are acceptable (dilutions must be made in a water miscible solvent) …

Extraction Extraction is the first step to separate the desired natural products from the raw materials. Extraction methods include solvent extraction, …

The phenol-chloroform DNA isolation method is one of the most classical and widely used methods to obtain a high molecular weight DNA such as human genomic DNA. This method is widely used in single nucleotide polymorphism (SNP) studies, gene quantification, gene amplification, DNA finger, and RFLP. DNA from blood cells, …

An extraction efficiency of E = 52% was achieved for solvent extraction and an extraction efficiency of E = 86% for reactive extraction at neutral pH. In the work of work of Utomo et al. [Citation 10] a higher extraction efficiency could be achieved, but the reactant D2EHPA used was not biocompatible compared to OLA in this work. Since ...

Objective . This study was to quantitatively synthesize data in randomized controlled trials (RCTs) of laparoscopic resection comparing natural orifice specimen extraction (NOSE) versus conventional laparoscopy (CL) in colorectal cancer. Methods . We identified eligible RCTs by searching seven electronic databases (PubMed, …

Patients with more than one tooth extraction received more often antibiotic treatment (10.7%) compared to those with single tooth extractions (χ 2 = 36; p < 0,001). Patients with more than one tooth extraction were older, however, younger patients received antibiotics more frequently (t = 28,774, p = 0.001). There was no relationship …

In this study, extraction of the M1 in the mandible during the developing dentition, at 8–11 years, had a significant mesialising and uprighting effect on the developing M3. This may improve future eruption and prevent pathology associated with impaction. This study was undertaken as an MClinDent research project with University College London.

Tau Tona est la plus grande mine d'or de la planète. Située à Carletonville, en Afrique du Sud, elle est profonde de 3,6 kilomètres, et ses multiples galeries s'étendent sur 800 kilomètres.

Extraction of premolars, as a routine orthodontic treatment for reducing protrusion or crowding of dental arch, has been widely used in the correction of severe tooth-arch discrepancy and, in certain cases, sagittal dysmorphosis. 8.9–13.4% of orthodontic treatments included four first premolar extractions, with an overall extraction frequency ...

Aujourd'hui, l'extraction industrielle se fait en plusieurs étapes : la roche extraite est finement broyée, puis plongée dans un bain approprié pour isoler l'or et le sulfure des …

Here we describe five extraction protocols including pre-treatment, bead-beating and/or Phenol:Chloroform:Isoamyl alcohol steps, applied to lung tissue samples …

1. Introduction. Wound healing is an essential aspect after dental extraction or any surgical procedure; it is defined as the complex and dynamic process of replacing devitalized and missing cellular structures and tissue layers [].Four essential phases constitute normal socket wound healing, i.e., hemostasis, inflammation, proliferation, and …

AbstractObjective:. To obtain through the use of discriminant analysis a relatively bias-free sample of extraction and nonextraction, Class I, equally susceptible to both treatments' malocclusions and to analyze retrospectively the soft tissue changes between the two different treatment groups.Materials and Methods:. The cephalometric, …

Introduction The peels from citrus fruit possess a large number of bioactive compounds, such as flavonoids and phenolic acids. As one of the most important groups …

0MM_PUR_CNF_MAA: n.a. DS only for Direct Access, 2181511: 0MM_PUR_DATA: n.a. DS only for Direct Access, 2181511: 0MM_PUR_MAA: n.a. DS only for Direct Access, 2181511: ... Therefore you will need to test the extraction of the DataSource via the ODP framework throughly and the usage is on your own risk. Hope …

Included a heating well spacing of 3.0 m, extraction pressure of 40 Kpa, extraction well influence radius of 4.35 m, extraction flow rate of 2.97 × 10-4 m 3 /s, and a theoretical number of 25 extraction wells (adjusted to 29 wells in practice), and the corresponding extraction well layout has been designed. The results can provide a …

1. Introduction. In recent years, optimization of extraction and purification techniques of multi components in natural products has been extensively studied and developed [1,2].However, when a method is applied for extracting or purifying the components of natural products, many factors need to be considered.